Section 465.APPENDIX A   Colisure P/A and Colisure Multiple Tube P/A

I.          Storage

            Store at 2.0º - 4.0º C.  Discard on or before expiration date on product package.

II.         Quality Control Testing

1A.      Colisure P/A

            Add 100 ml of sterile laboratory pure water to the medium in one 125 ml bottle.  Shake to dissolve.  Transfer a 20 ml aliquot to each of 4 25 ml sterile glass tubes.

2A.      Colisure Multiple Tube P/A

            Rehydrate 3 vials with 20 ml of sterile laboratory pure water.  Shake to dissolve.

B.         Test by inoculating with E. coli, a total coliform other than E. coli, and a non-coliform.  If Pseudomonas is used as the non-coliform, use a nonfluorescent species.  Use the uninoculated tube as an additional control.

C.        Incubate at 35º + 0.5º C for 28 hours and observe for the following results:

III.       Test Procedure

A.        Allow medium and samples to reach room temperature.

B.         Add a 100 ml water sample to the bottle containing the dehydrated Colisure medium for a single bottle test.  Then recap the bottle.

            OR

            Add a 20 ml water sample to each of the 5 tubes containing the dehydrated  Colisure medium for a multiple tube test.  Then recap each tube.

C.        Shake and invert the bottle or tube(s) to thoroughly mix the contents. The solution will appear yellow with a fine precipitate.

D.        Incubate the bottle or tube(s) at 35º ± 0.5º C for 28 hours.

IV.       Interpretation

A.        Check each tube or bottle visually for color.

B.         Examine each positive result for fluorescence using a long wave (366 nm) ultraviolet light.  If the solution glows a uniform, bright, light blue throughout the bottle or tube, fluorescence is present.  This indicates the presence of E. coli.